RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection.
Although dideoxy terminated sequencing of RNA, using reverse transcriptase and oligodeoxynucleotide primers, is now a well established method, the accuracy is limited by sequence ambiguities due to unspecific chain termination events. A protocol is described which circumvents these ambiguities by us...
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| Publicado en: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1990
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330340/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1690393/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.4.879 |
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