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RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection.

Although dideoxy terminated sequencing of RNA, using reverse transcriptase and oligodeoxynucleotide primers, is now a well established method, the accuracy is limited by sequence ambiguities due to unspecific chain termination events. A protocol is described which circumvents these ambiguities by us...

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Hlavní autor: Bauer, G J
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC330340/
https://ncbi.nlm.nih.gov/pubmed/1690393
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