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Participation of glutamic acid 23 of T4 endonuclease V in the beta-elimination reaction of an abasic site in a synthetic duplex DNA.

T4 endonuclease V catalyzes the hydrolysis of the glycosyl bond of a thymine dimer in a DNA duplex and the cleavage of the 3'-phosphate by beta-elimination. We have previously identified a catalytic site for the first reaction (pyrimidine dimer-glycosylase activity) by systematic mutagenesis (D...

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Autori principali: Hori, N, Doi, T, Karaki, Y, Kikuchi, M, Ikehara, M, Ohtsuka, E
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC334229/
https://ncbi.nlm.nih.gov/pubmed/1357629
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