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Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR.
Thermus aquaticus (Taq) DNA polymerase was used to measure the extension efficiency for all configurations of matched and mismatched base pairs at template-primer 3'-termini. The transition mispairs, A(primer).C, C.A, G.T, and T.G were extended 10(-3) to 10(-4)-fold less efficiently than their...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1992
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| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334186/ https://ncbi.nlm.nih.gov/pubmed/1408758 |
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