An Introduction to Worm Lab: from Culturing Worms to Mutagenesis

This protocol describes procedures to maintain nematodes in the laboratory and how to mutagenize them using two alternative methods: ethyl methane sulfonate (EMS) and 4, 5', 8-trimethylpsoralen combined with ultraviolet light (TMP/UV). Nematodes are powerful biological systems for genetics stud...

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Bibliographic Details
Main Authors: Chaudhuri, Jyotiska, Parihar, Manish, Pires-daSilva, Andre
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2011
Subjects:
Basic Protocols
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3341104/
https://ncbi.nlm.nih.gov/pubmed/21248706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2293
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