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Biotinylation of lysine method identifies acetylated histone H3 lysine 79 in Saccharomyces cerevisiae as a substrate for Sir2

Although the biological roles of many members of the sirtuin family of lysine deacetylases have been well characterized, a broader understanding of their role in biology is limited by the challenges in identifying new substrates. We present here an in vitro method that combines biotinylation and mas...

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Detalhes bibliográficos
Main Authors: Bheda, Poonam, Swatkoski, Stephen, Fiedler, Katherine L., Boeke, Jef D., Cotter, Robert J., Wolberger, Cynthia
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3341040/
https://ncbi.nlm.nih.gov/pubmed/22474337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1121471109
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