In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells.

We describe a process for the identification of mRNAs within single cells, as demonstrated with the immunoglobulin (Ig) variable region (V) genes of two mouse hybridoma cell lines and the bcr-abl fusion gene of the human K562 myeloid leukaemia line. The cells were fixed and permeabilised, the mRNA r...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Embleton, M J, Gorochov, G, Jones, P T, Winter, G
Format: Artigo
Language:Inglês
Published: Oxford University Press 1992
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334055/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1508667/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.15.3831
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