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Expression and characterisation of the korB gene product from the Streptomyces lividans plasmid pIJ101 in Escherichia coli and determination of its binding site on the korB and kilB promoters.

A 0.5kb Spel-BclI fragment containing the pIJ101 korB ORF was cloned into pUC8 under the control of the lacZ promoter, creating pQR206. In vitro coupled transcription-translation of pQR206 identified a protein product of approximately 10kDa, which corresponds to the predicted molecular weight deduce...

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Zaman, S, Richards, H, Ward, J
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 1992
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334020/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1641335/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.14.3693
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