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Expression and characterisation of the korB gene product from the Streptomyces lividans plasmid pIJ101 in Escherichia coli and determination of its binding site on the korB and kilB promoters.

A 0.5kb Spel-BclI fragment containing the pIJ101 korB ORF was cloned into pUC8 under the control of the lacZ promoter, creating pQR206. In vitro coupled transcription-translation of pQR206 identified a protein product of approximately 10kDa, which corresponds to the predicted molecular weight deduce...

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Bibliografische gegevens
Hoofdauteurs: Zaman, S, Richards, H, Ward, J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1992
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC334020/
https://ncbi.nlm.nih.gov/pubmed/1641335
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