A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells in culture.
We describe a rapid and general method for isolating DNA-binding proteins in high yield from purified nuclei of animal cells. The method has been tested for the isolation of a series of different DNA-binding activities including those of transcription factors PTF1 and SP1. The rationale consists of...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1992
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334001/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1641323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.14.3555 |
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