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A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells in culture.
We describe a rapid and general method for isolating DNA-binding proteins in high yield from purified nuclei of animal cells. The method has been tested for the isolation of a series of different DNA-binding activities including those of transcription factors PTF1 and SP1. The rationale consists of...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1992
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334001/ https://ncbi.nlm.nih.gov/pubmed/1641323 |
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