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A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells in culture.

We describe a rapid and general method for isolating DNA-binding proteins in high yield from purified nuclei of animal cells. The method has been tested for the isolation of a series of different DNA-binding activities including those of transcription factors PTF1 and SP1. The rationale consists of...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Hagenbüchle, O, Wellauer, P K
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: 1992
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC334001/
https://ncbi.nlm.nih.gov/pubmed/1641323
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