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Fiber Laser Based Two-Photon FRET Measurement of Calmodulin and mCherry-E(0)GFP Proteins
The speed and accuracy of Förster Resonance Energy Transfer (FRET) measurements can be improved by rapidly alternating excitation wavelengths between the donor and acceptor fluorophore. We demonstrate FRET efficiency measurements based on a fiber laser and photonic crystal fiber as the source for tw...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3339282/ https://ncbi.nlm.nih.gov/pubmed/22213386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.22002 |
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