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DNA hairpin loops in solution. Correlation between primary structure, thermostability and reactivity with single-strand-specific nuclease from mung bean.

Hairpin structures formed by seven DNA inverted repeats have been studied by PAGE, UV(CD)-spectroscopy and nuclease cleavage. The hairpins consisted of (CG)3 stems and loops of 2, 3 and 4 residues. Thermal stabilities (Tm) have been determined in low and high ionic strength buffers, where the hairpi...

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Detalhes bibliográficos
Main Authors: Xodo, L E, Manzini, G, Quadrifoglio, F, van der Marel, G, van Boom, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC333908/
https://ncbi.nlm.nih.gov/pubmed/2027758
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