Expression of tetanus toxin fragment C in yeast: gene synthesis is required to eliminate fortuitous polyadenylation sites in AT-rich DNA.

Fragment C is a non-toxic 50 kDa fragment of tetanus toxin which is a candidate subunit vaccine against tetanus. The AT-rich Clostridium tetani DNA encoding fragment C could not be expressed in Saccharomyces cerevisiae due to the presence of several fortuitous polyadenylation sites which gave rise t...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Romanos, M A, Makoff, A J, Fairweather, N F, Beesley, K M, Slater, D E, Rayment, F B, Payne, M M, Clare, J J
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1991
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC333902/
https://ncbi.nlm.nih.gov/pubmed/2027754
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