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A novel fluorescent assay for sucrose transporters

BACKGROUND: We have developed a novel assay based on the ability of type I sucrose uptake transporters (SUTs) to transport the fluorescent coumarin β-glucoside, esculin. Budding yeast (Saccharomyces cerevisiae) is routinely used for the heterologous expression of SUTs and does not take up esculin. R...

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Bibliografski detalji
Glavni autori: Gora, Peter J, Reinders, Anke, Ward, John M
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3337809/
https://ncbi.nlm.nih.gov/pubmed/22475854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-8-13
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