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A novel fluorescent assay for sucrose transporters
BACKGROUND: We have developed a novel assay based on the ability of type I sucrose uptake transporters (SUTs) to transport the fluorescent coumarin β-glucoside, esculin. Budding yeast (Saccharomyces cerevisiae) is routinely used for the heterologous expression of SUTs and does not take up esculin. R...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3337809/ https://ncbi.nlm.nih.gov/pubmed/22475854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-8-13 |
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