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Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2.

The restriction-modification system HgiDI from Herpetosiphon giganteus strain Hpa2 has been cloned in E. coli in a two-step procedure. Selection of the methyltransferase (M.HgiDI) gene in vitro was performed using the heterologous restriction endonuclease AhaII, an isoschizomer of Acyl and HgiDI (GR...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Düsterhöft, A, Erdmann, D, Kröger, M
פורמט: Artigo
שפה:Inglês
יצא לאור: 1991
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC333779/
https://ncbi.nlm.nih.gov/pubmed/2020544
תגים: הוספת תג
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