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Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites.

A series of computer simulations of gel patterns assuming non-cooperative binding of a protein to two targets on the same DNA fragment was performed and applied to interprete gel mobility shift experiments of Tet repressor-tet operator binding. While a high binding affinity leads to the expected dis...

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Библиографические подробности
Главные авторы: Kleinschmidt, C, Tovar, K, Hillen, W
Формат: Artigo
Язык:Inglês
Опубликовано: 1991
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC333775/
https://ncbi.nlm.nih.gov/pubmed/2020542
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