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Selection for Tn10 Tet Repressor Binding to Tet Operator in Escherichia Coli: Isolation of Temperature-Sensitive Mutants and Combinatorial Mutagenesis in the DNA Binding Motif

We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacI and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be...

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Sonraí Bibleagrafaíochta
Main Authors: Wissmann, A., Wray-Jr, L. V., Somaggio, U., Baumeister, R., Geissendorfer, M., Hillen, W.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1991
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1204461/
https://ncbi.nlm.nih.gov/pubmed/1649065
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