Laddar...
Selection for Tn10 Tet Repressor Binding to Tet Operator in Escherichia Coli: Isolation of Temperature-Sensitive Mutants and Combinatorial Mutagenesis in the DNA Binding Motif
We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacI and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be...
Sparad:
| Huvudupphovsmän: | , , , , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1991
|
| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1204461/ https://ncbi.nlm.nih.gov/pubmed/1649065 |
| Taggar: |
Lägg till en tagg
Inga taggar, Lägg till första taggen!
|