Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

The BamHI restriction modification system was previously cloned into E. coli and maintained with an extra copy of the methylase gene on a high copy vector (Brooks et al., (1989) Nucl. Acids Res. 17, 979-997). The nucleotide sequence of a 3014 bp region containing the endonuclease (R) and methylase (...

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Main Authors: Brooks, J E, Nathan, P D, Landry, D, Sznyter, L A, Waite-Rees, P, Ives, C L, Moran, L S, Slatko, B E, Benner, J S
Format: Artigo
Jezik:Inglês
Izdano: 1991
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC333720/
https://ncbi.nlm.nih.gov/pubmed/1901989
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