Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

The BamHI restriction modification system was previously cloned into E. coli and maintained with an extra copy of the methylase gene on a high copy vector (Brooks et al., (1989) Nucl. Acids Res. 17, 979-997). The nucleotide sequence of a 3014 bp region containing the endonuclease (R) and methylase (...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Brooks, J E, Nathan, P D, Landry, D, Sznyter, L A, Waite-Rees, P, Ives, C L, Moran, L S, Slatko, B E, Benner, J S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1991
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1901989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.4.841
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