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Phosphorothioate-containing RNAs show mRNA activity in the prokaryotic translation systems in vitro.
Phosphorothioate-containing RNAs were generated by transcription of coliphage T7 DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) and T7 RNA polymerase. RNAs in which a single nucleotide was substituted by the corresponding nucleoside phosphorothioate functioned as mR...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1991
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333646/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2011526/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.3.547 |
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