Učitavanje...

Cytosine methylated DNA synthesized by Taq polymerase used to assay methylation sensitivity of restriction endonuclease HinfI.

We have studied the resistance of cytosine methylated DNA to digestion by the restriction endonuclease HinfI, using a simple PCR procedure to synthesize DNA of known sequence in which every cytosine is methylated at the 5 position. We find that HinfI cannot digest cytosine methylated DNA at the conc...

Cijeli opis

Spremljeno u:
Bibliografski detalji
Glavni autori: Colasanti, J, Sundaresan, V
Format: Artigo
Jezik:Inglês
Izdano: 1991
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC333607/
https://ncbi.nlm.nih.gov/pubmed/2014176
Oznake: Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!