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Cytosine methylated DNA synthesized by Taq polymerase used to assay methylation sensitivity of restriction endonuclease HinfI.

We have studied the resistance of cytosine methylated DNA to digestion by the restriction endonuclease HinfI, using a simple PCR procedure to synthesize DNA of known sequence in which every cytosine is methylated at the 5 position. We find that HinfI cannot digest cytosine methylated DNA at the conc...

詳細記述

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書誌詳細
主要な著者: Colasanti, J, Sundaresan, V
フォーマット: Artigo
言語:Inglês
出版事項: 1991
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC333607/
https://ncbi.nlm.nih.gov/pubmed/2014176
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