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Covalent Bond-Based Immobilization Approaches for Single-Molecule Fluorescence

INH: We report two new approaches, using click-chemistry and disulfide bond bridges, to surface-immobilize nucleic acids for single-molecule fluorescence experiments using covalent bonds and self-assembled monolayers. Both approaches are specific and yield comparable results to the avidin-biotin lin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Alemán, Elvin A., Pedini, Heidi S., Rueda, David
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3331594/
https://ncbi.nlm.nih.gov/pubmed/19911404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.200900640
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