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Ribonucleoprotein Immunoprecipitation (RNP-IP): A Direct in vivo Analysis of microRNA-Targets
We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRN...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3329753/ https://ncbi.nlm.nih.gov/pubmed/20564179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcb.22562 |
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