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Redesigning the NEDD8 pathway with a bacterial genetic screen for ubiquitin-like molecule transfer
Pathways of ubiquitin-like (UBL) molecule transfer regulate a myriad of cellular cascades. Here we report a high-throughput assay that correlates catalytic human-NEDD8 transfer to bacterial survival. The assay was utilized to screen mutant NEDD8 and NEDD8-activating enzyme (NAE) libraries to enginee...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3329750/ https://ncbi.nlm.nih.gov/pubmed/22391419 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.02.036 |
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