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Sequence-specific affinity selection of mammalian splicing complexes.
Antisense oligonucleotides made of 2'-OMe RNA are shown to bind specifically and efficiently to targeted sites on pre-mRNA substrates, allowing affinity selection of splicing complexes using streptavidin/biotin chromatography. The position of probe binding to the pre-mRNA influences which type...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1990
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332875/ https://ncbi.nlm.nih.gov/pubmed/2147994 |
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