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Magnetic-based purification of untouched mouse germinal center B cells for ex vivo manipulation and biochemical analysis

Detailed biochemical analysis of unmanipulated germinal center (Gc) B cells has not been achieved. previously, we designed and used a simple, economical and new magnetic bead separation scheme for the purification of ‘untouched’ mature Gc and non-Gc B cells from the spleens of immunized mice and rep...

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Detalles Bibliográficos
Main Authors: Cato, Matthew H, Yau, Irene W, Rickert, Robert C
Formato: Artigo
Idioma:Inglês
Publicado: 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3326360/
https://ncbi.nlm.nih.gov/pubmed/21720310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.344
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