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Magnetic-based purification of untouched mouse germinal center B cells for ex vivo manipulation and biochemical analysis
Detailed biochemical analysis of unmanipulated germinal center (Gc) B cells has not been achieved. previously, we designed and used a simple, economical and new magnetic bead separation scheme for the purification of ‘untouched’ mature Gc and non-Gc B cells from the spleens of immunized mice and rep...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3326360/ https://ncbi.nlm.nih.gov/pubmed/21720310 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.344 |
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