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Ligation-independent cloning of PCR products (LIC-PCR).

A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes...

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Shranjeno v:
Bibliografske podrobnosti
Main Authors: Aslanidis, C, de Jong, P J
Format: Artigo
Jezik:Inglês
Izdano: 1990
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC332407/
https://ncbi.nlm.nih.gov/pubmed/2235490
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