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Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Several ligation-independent cloning methods have been developed that offer advantages for construction of recombinant plasmids, including high efficiency while minimizing cloning artifacts. Here we report new plasmid vectors that use the nicking endonuclease Nt.BspQI to generate extended single str...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2011
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3220417/ https://ncbi.nlm.nih.gov/pubmed/21854804 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2011.07.007 |
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