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Construction of new cloning, lacZ reporter and scarless-markerless suicide vectors for genetic studies in Aggregatibacter actinomycetemcomitans

To elucidate the putative function of a gene, effective tools are required for genetic characterization that facilitate its inactivation, deletion or modification on the bacterial chromosome. In the present study, the nucleotide sequence of the Escherichia coli/Aggregatibacter actinomycetemcomitans...

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Detalhes bibliográficos
Main Authors: Juárez-Rodríguez, María Dolores, Torres-Escobar, Ascención, Demuth, Donald R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3650090/
https://ncbi.nlm.nih.gov/pubmed/23353051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2013.01.002
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