Ligation-independent cloning of PCR products (LIC-PCR).
A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1990
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332407/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2235490/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.20.6069 |
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