Bivalent Carbohydrate Binding Is Required for Biological Activity of Clitocybe nebularis Lectin (CNL), the N,N′-Diacetyllactosediamine (GalNAcβ1–4GlcNAc, LacdiNAc)-specific Lectin from Basidiomycete C. nebularis

Lectins are carbohydrate-binding proteins that exert their biological activity by binding to specific cell glycoreceptors. We have expressed CNL, a ricin B-like lectin from the basidiomycete Clitocybe nebularis in Escherichia coli. The recombinant lectin, rCNL, agglutinates human blood group A eryth...

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Detalhes bibliográficos
Main Authors: Pohleven, Jure, Renko, Miha, Magister, Špela, Smith, David F., Künzler, Markus, Štrukelj, Borut, Turk, Dušan, Kos, Janko, Sabotič, Jerica
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Glycobiology and Extracellular Matrices
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3323013/
https://ncbi.nlm.nih.gov/pubmed/22298779
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.317263
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