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Kinetics of Precursor Interactions with the Bacterial Tat Translocase Detected by Real-time FRET

The Escherichia coli twin-arginine translocation (Tat) system transports fully folded and assembled proteins across the inner membrane into the periplasmic space. Traditionally, in vitro protein translocation studies have been performed using gel-based transport assays. This technique suffers from l...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Whitaker, Neal, Bageshwar, Umesh K., Musser, Siegfried M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3322843/
https://ncbi.nlm.nih.gov/pubmed/22315217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.324525
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