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Bacterial Sec Protein Transport Is Rate-limited by Precursor Length: A Single Turnover Study
An in vitro real-time single turnover assay for the Escherichia coli Sec transport system was developed based on fluorescence dequenching. This assay corrects for the fluorescence quenching that occurs when fluorescent precursor proteins are transported into the lumen of inverted membrane vesicles....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Cell Biology
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2754939/ https://ncbi.nlm.nih.gov/pubmed/19656854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E09-01-0075 |
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