Solid phase in vitro mutagenesis using plasmid DNA template.
Site-specific mutagenesis was accomplished using a solid support to generate single stranded vector and insert fragments which can be used to form gap-duplex plasmids through flanking, complementary double stranded regions. More than 80% mutants were obtained in both a single and a double primer app...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1990
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332130/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2205837/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.17.5107 |
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