Subtractive hybridization system using single-stranded phagemids with directional inserts.
We describe a subtractive hybridization protocol which is designed to permit subtractions between cDNA libraries. The method uses single-stranded phagemids with directional inserts as both the driver and the target. We modified the M13 phagemid vector pBluescript for the directional cDNA cloning and...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1990
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331957/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2168539/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.16.4833 |
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