Subtractive hybridization system using single-stranded phagemids with directional inserts.

We describe a subtractive hybridization protocol which is designed to permit subtractions between cDNA libraries. The method uses single-stranded phagemids with directional inserts as both the driver and the target. We modified the M13 phagemid vector pBluescript for the directional cDNA cloning and...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Rubenstein, J L, Brice, A E, Ciaranello, R D, Denney, D, Porteus, M H, Usdin, T B
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1990
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331957/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2168539/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.16.4833
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