Subtractive hybridization system using single-stranded phagemids with directional inserts.

We describe a subtractive hybridization protocol which is designed to permit subtractions between cDNA libraries. The method uses single-stranded phagemids with directional inserts as both the driver and the target. We modified the M13 phagemid vector pBluescript for the directional cDNA cloning and...

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Bibliographic Details
Main Authors: Rubenstein, J L, Brice, A E, Ciaranello, R D, Denney, D, Porteus, M H, Usdin, T B
Format: Artigo
Language:Inglês
Published: 1990
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC331957/
https://ncbi.nlm.nih.gov/pubmed/2168539
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