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Localized Hydration in Lyophilized Myoglobin by Hydrogen-Deuterium Exchange Mass Spectrometry. 1. Exchange Mapping

The local effects of hydration on myoglobin (Mb) in solid matrices containing mannitol or sucrose (1:1 w/w, protein:additive) were mapped using hydrogen-deuterium exchange with mass spectrometric analysis (HDX-MS) at 5°C and compared to solution controls. Solid powders were exposed to D(2)O(g) at co...

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Autori principali: Sophocleous, Andreas M., Zhang, Jun, Topp, Elizabeth M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3319197/
https://ncbi.nlm.nih.gov/pubmed/22352965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/mp3000088
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