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Localized Hydration in Lyophilized Myoglobin by Hydrogen-Deuterium Exchange Mass Spectrometry. 1. Exchange Mapping
The local effects of hydration on myoglobin (Mb) in solid matrices containing mannitol or sucrose (1:1 w/w, protein:additive) were mapped using hydrogen-deuterium exchange with mass spectrometric analysis (HDX-MS) at 5°C and compared to solution controls. Solid powders were exposed to D(2)O(g) at co...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3319197/ https://ncbi.nlm.nih.gov/pubmed/22352965 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/mp3000088 |
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