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Measuring Binding of Protein to Gel-Bound Ligands Using Magnetic Levitation

This paper describes the use of magnetic levitation (MagLev) to measure the association of proteins and ligands. The method starts with diamagnetic gel beads that are functionalized covalently with small molecules (putative ligands). Binding of protein to the ligands within the bead causes a change...

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Autors principals: Shapiro, Nathan D., Mirica, Katherine A., Soh, Siowling, Phillips, Scott T., Taran, Olga, Mace, Charles R., Shevkoplyas, Sergey S., Whitesides, George M.
Format: Artigo
Idioma:Inglês
Publicat: 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3319098/
https://ncbi.nlm.nih.gov/pubmed/22364170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja211788e
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