Attenuation-Based Dual-Fluorescent-Protein Reporter for Screening Translation Inhibitors

A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of t...

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Détails bibliographiques
Auteurs principaux: Osterman, Ilya A., Prokhorova, Irina V., Sysoev, Vasily O., Boykova, Yulia V., Efremenkova, Olga V., Svetlov, Maxim S., Kolb, Vyacheslav A., Bogdanov, Alexey A., Sergiev, Petr V., Dontsova, Olga A.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2012
Sujets:
Analytical Procedures
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3318315/
https://ncbi.nlm.nih.gov/pubmed/22252829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.05395-11
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