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Isolation and in vitro expression of the ricin A-chain gene: effect of deletions on biological activity.
The gene encoding the ricin A-chain was isolated and subcloned into an in vitro expression vector downstream from the SP6 promotor. mRNA encoding the A-chain strongly inhibited the translational activity of reticulocyte lysates. The inhibition correlated with glycosylase activity on rRNA, and could...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1989
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331830/ https://ncbi.nlm.nih.gov/pubmed/2922291 |
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