Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles

Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics approach to analyzing clathrin-coated vesicles (CCVs) from HeLa cells. siRNA knockdown of coat components and different fractionation protoc...

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Autors principals: Borner, Georg H.H., Antrobus, Robin, Hirst, Jennifer, Bhumbra, Gary S., Kozik, Patrycja, Jackson, Lauren P., Sahlender, Daniela A., Robinson, Margaret S.
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3317806/
https://ncbi.nlm.nih.gov/pubmed/22472443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201111049
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