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Cloning the BamHI restriction modification system.
BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Although within the clone the methylase and endonucl...
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| Hauptverfasser: | , , , , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1989
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331717/ https://ncbi.nlm.nih.gov/pubmed/2537955 |
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