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A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines

Mass spectrometry-based proteomics increasingly relies on relative or absolute quantification. In relative quantification, stable isotope based methods often allow mixing at early stages of sample preparation, whereas for absolute quantification this has generally required recombinant expression of...

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Autors principals: Zeiler, Marlis, Straube, Werner L., Lundberg, Emma, Uhlen, Mathias, Mann, Matthias
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Biochemistry and Molecular Biology 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3316735/
https://ncbi.nlm.nih.gov/pubmed/21964433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O111.009613
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