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A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines

Mass spectrometry-based proteomics increasingly relies on relative or absolute quantification. In relative quantification, stable isotope based methods often allow mixing at early stages of sample preparation, whereas for absolute quantification this has generally required recombinant expression of...

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Библиографические подробности
Главные авторы: Zeiler, Marlis, Straube, Werner L., Lundberg, Emma, Uhlen, Mathias, Mann, Matthias
Формат: Artigo
Язык:Inglês
Опубликовано: The American Society for Biochemistry and Molecular Biology 2012
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3316735/
https://ncbi.nlm.nih.gov/pubmed/21964433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O111.009613
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