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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Membrane proteins such as receptors and ion channels undergo active trafficking in neurons, which are highly polarised and morphologically complex. This directed trafficking is of fundamental importance to deliver, maintain or remove synaptic proteins. Super-ecliptic pHluorin (SEP) is a pH-sensitive...

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Detalhes bibliográficos
Main Authors: Hildick, Keri L., González-González, Inmaculada M., Jaskolski, Frédéric, Henley, Jeremy. M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315441/
https://ncbi.nlm.nih.gov/pubmed/22395448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3747
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