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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Membrane proteins such as receptors and ion channels undergo active trafficking in neurons, which are highly polarised and morphologically complex. This directed trafficking is of fundamental importance to deliver, maintain or remove synaptic proteins. Super-ecliptic pHluorin (SEP) is a pH-sensitive...

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Auteurs principaux: Hildick, Keri L., González-González, Inmaculada M., Jaskolski, Frédéric, Henley, Jeremy. M.
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2012
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315441/
https://ncbi.nlm.nih.gov/pubmed/22395448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3747
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